Olomouc scientists have developed new substances that can protect human skin from the harmful effects of ultraviolet radiation. They derived the compounds from kinetin – one of the cytokinins – plant hormones that affect cell division. The effects of substances were reported by the researchers at Palacký University Olomouc and the Olomouc workplace of the Institute of Experimental Botany (IEB) of the Academy of Sciences of the Czech Republic, a part of the Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), in a study published in the European Journal of Medicinal Chemistry. Small molecules could find their use in cosmetics and medicine.
The development of small molecules derived from cytokinins has been in focus of IEB and UP scientists for a long time, some of these derivatives have already been applied in practice. This time, they focused on kinetin-based substances and, in collaboration with colleagues at the Faculty of Medicine UPOL, examined their effects on human skin cells.
“For the first time, we have found that cytokinins and their derivatives can protect skin cells from the harmful effects of both types of ultraviolet radiation (UVB and UVA). This is new information, scientists have not dealt with this question before. It is also important to find that our substances support the natural defense of skin cells,” said the first author of the paper Martin Hönig from CRH. The research is concurrently performed within Martin Hönig´s doctoral thesis at the Faculty of Science, Palacký University Olomouc.
Over the past five years, the team has been making various changes in kinetin molecule and has been monitoring the impact of the structure change on molecule biological activity. Various derivatives were then tested on human skin cells in vitro, i.e. under laboratory conditions. “We first confirmed that the substances are not phototoxic, that they do not become dangerous under UV radiation. We have subsequently shown that they even contribute to protecting skin cells by the modulation of their natural defense,” said project coordinator Lucie Plíhalová.
Beneficial effects have been also confirmed in vivo. Organism Caenorhabditis elegans was exposed to the action of new derivatives. C. elegans is a soil nematode serving to scientists as a model organism for the study of stress and aging. A number of substances have surprisingly similar effects to mammals when tested on this organism. “We have found that our substances protect the nematode from the oxidative stress that we artificially initiated. It protects not only cells in tissue culture but also the whole organism against stress,” added Plíhalová. Research has followed up previous studies and is still carried out. Currently, researchers are looking more accurately into the mechanism of action of a series of substances they have developed. The substances have been protected by patent application and a relatively large cosmetic company is now testing a selected derivative for further use in cosmetic industry.